Effect of Serum Containing Rhizoma Chuanxiong on Proliferation and Differentiation of Mouse Embryonic Stem Cells
10.13359/j.cnki.gzxbtcm.2017.03.025
- VernacularTitle:川芎含药血清对小鼠胚胎干细胞增殖和分化的影响
- Author:
Deju JIANG
;
Yi LUO
;
Qian BAO
;
Quan XIA
- Keywords:
Rhizoma Chuanxiong/pharmacology;
embryonic stem cells /pathology;
cell proliferation;
cell differentiation;
cell culture;
gene expression regulation
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(3):401-404
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of serum containing Rhizoma Chuanxiong on the proliferation and differentiation of mouse embryos stem cells(ESCs).Methods Mouse ESCs were co-cultured with serum containing Rhizoma Chuanxiong.The proliferation of ESCs was detected by CCK-8 method.The expression level of specific gene beta-myosin heavy chain (β-MHC) in cardiac myoblasts was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results Compared with the blank rat serum group and the blank fetal bovine serum group,the differences of activities of ESCs in serum containing Rhizoma Chuanxiong group were in significant(P ＞0.05) and the expression level of specific gene β-MHC in cardiac myoblasts was increased (P ＜ 0.05).Conclusion Serum containing Rhizoma Chuanxiong can promote the differentiation of ESCs into cardiac myoblasts.
