Correlation of HPLC Fingerprint Chromatograms of Cinnamomi Ramulus Formula Granules, Decoction Pieces and Water Decoction
10.3969/j.issn.1005-5304.2017.06.022
- VernacularTitle:桂枝配方颗粒、饮片、水煎剂HPLC指纹图谱相关性研究
- Author:
Xiaoya WANG
;
Yaqin ZHEN
;
Xinguo WANG
;
Guozhi JIANG
;
Liying NIU
- Keywords:
Cinnamomi Ramulus formula granules;
decoction pieces;
water decoction;
HPLC;
fingerprint chromatogram;
correlation;
cinnamic acid
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(6):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the correlation of fingerprint chromatograms of Cinnamomi Ramulus formula granules, decoction pieces and water decoction by HPLC; To investigate the difference of main chemical constituents among different forms. Methods The Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used with mobile phase of acetonitrile and 0.1% phosphoric acid at the flow rate of 1.0 mL/min, with detection wave of 280 nm and temperature of 30 ℃. The detection of 10 batches of Cinnamomi Ramulus formula granules, 10 batches of decoction pieces and 10 batches of water decoction were established respectively. Results Totally 12 peaks in the HPLC fingerprint chromatogram from 10 batches of formula granules could be tracked in the water decoction; 10 peaks in the HPLC fingerprint chromatogram could be tracked in the decoction pieces. Three components, such as protocatechuic acid, coumarin and cinnamic acid were verified. Conclusion The main chemical components of Cinnamomi Ramulus formula granules and water decoction are basically the same, and the common component contents have similar proportion.
