Comparison of Two Kinds of Rat Model for Slow Transit Constipation and Expression of Aquaporins in Them

VernacularTitle:两种不同慢传输便秘大鼠模型构建方法比较及对水通道蛋白的影响
Author: Tianli JI ; Haihua QIAN
Keywords: slow transit constipation; model; aquaporin; rats
From: Chinese Journal of Rehabilitation Theory and Practice 2017;23(5):529-533
CountryChina
Language:Chinese
Abstract: Objective To compare the rat model of slow transit constipation (STC) established with gavage or feeding compound di-phenoxylate. Methods Forty Sprague-Dawley rats were randomly divided into gavage control group (A), gavage group (B), feeding control group (C) and feeding group (D) equally. Group B was given compound diphenoxylate suspension by gavage for 14 days while group A ac-cepted isodose normal saline. Group D were given feed containing compound diphenoxylate for 120 days while group C accepted normal feed. Carbon ink was poured into stomachs to observe the propelling rate after modeling, and the content of aquaporin 3 and aquaporin 4 were detected with immunohistochemistry. Results There were two rats died in group A and group B, respectively. The carbon ink propel-ling rate was lower in group D than in group B (P<0.05). Both aquaporin 3 and aquaporin 4 were more in group D than in group B (P<0.05). Conclusion STC rat model can be established by eating diphenoxylate feed for 120 days, which is safer and more stable than that of gavage for 14 days. Aquaporins in colons may play a role in onset and development of STC.