Diatom Detection Using Enzyme Combined with Strong Acid Digestion Method
10.3969/j.issn.1004-5619.2017.02.012
- VernacularTitle:酶消化联合强酸消化法的硅藻检验
- Author:
Jian YAO
;
Weijun KAN
- Keywords:
forensic pathology;
drow ning;
diatom;
strong acid digestion m ethod;
enzym atic digestion m ethod
- From:Journal of Forensic Medicine
2017;33(2):165-167
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o detect diatom in the organs of drow ners by enzym e com bined w ith strong acid digestion m ethod, and evaluate its application value. Methods A total of 40 cases w hich have been identified as drow ning in local region w ere collected. Sam ples of the lung, liver, kidney, and the w ater of the scene w ere also gathered from each case. Strong acid digestion m ethod, enzym e com bined w ith strong acid digestion m ethod, and enzym ic digestion m ethod w ere respectively perform ed to detect the diatom in the sam ples. T he com parative analysis w as m ade on digestion tim e, digestive pow er and de-tection rate of diatom , etc. Results E nzym e com bined w ith strong acid digestion m ethod w as significant-ly better than enzym ic digestion m ethod on digestion tim e and digestive pow er;enzym e com bined w ith strong acid digestion m ethod w ere obviously superior to strong acid digestion m ethod on the detection rate of diatom . Conclusion E nzym e com bined w ith strong acid digestion m ethod com bines the advan-tages of strong acid digestion m ethod and enzym ic digestion m ethod. It has the characters of operation safety w ith little pollution to environm ent, w hich is w orthy of further popularization and practice.
