Effect of Sinomenine on Expression of COX2, α7nAChR and A2A in A549 Cells
10.13359/j.cnki.gzxbtcm.2017.03.020
- VernacularTitle:青藤碱对肺癌细胞A549中COX2、α7nAChR和A2A表达的影响
- Author:
Jiexiu WU
;
Shasha BAI
;
Bingbing XIE
;
Lang YI
;
Ping QIU
;
Yan DONG
;
Peixun WANG
- Keywords:
sinomenine/pharmacology;
lung cancer/TCD therapy;
COX2;
α7nAChR;
A2A;
gene expression regulation;
cell proliferation;
cell culture
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(3):381-386
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of sinomenine (SIN) on the expression of cyclooxygenase (COX2),alpha-7 nicotinic acetylcholine receptor(α7nAChR) and adenosine receptor(A2A) in A549 cells,and to explore the relative mechanism for cell proliferation.Methods The effect of SIN and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) on the proliferation of A549 cells was determined by methyl thiazolyl tetrazolium (MTT) assay.The effect of SIN and NNK on the migration of A549 cells was detected by cell wound scratch assay.The effect of SIN and NNK on COX2 expression in A549 cells was determined by Western blotting method.The effect of SIN and NNK on the expression of α7nAChR and A2A mRNA and protein was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting method.Results NNK increased the proliferation and migration of A549 cells,while SIN inhibited the proliferation and migration of A549 cells.COX2 expression level was increased in NNK group but was decreased in SIN group.The expression levels of α7nAChR and A2A were up-regulated in NNK group but were down-regulated in SIN group.Conclusion SIN plays a role in inhibiting the proliferation and migration of A549 cells by suppressing COX2 expression.SIN has an inhibitory effect on the expression of α7nAChR and A2A.
