Effect of Kidney-reinforcing, Blood-activating and Phlegm-resolving Therapy on Left Ventricular Fibrosis of Spontaneously Hypertensive Rats
10.13359/j.cnki.gzxbtcm.2017.03.024
- VernacularTitle:补肾活血化痰法对自发性高血压大鼠左心室纤维化的影响
- Author:
Qiong WANG
;
Shaoxiang XIAN
;
Zhongqi YANG
;
Bingqing LYU
;
Zheng ZHOU
;
Jiao DUAN
;
Yaqin TANG
- Keywords:
ventricular fibrosis;
hypertension/TCD therapy;
Smad3;
myocardium/pathology;
gene expression regulation;
disease models,animal;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(3):397-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the therapeutic mechanism of kidney-reinforcing,blood-activating and phlegmresolving therapy for left ventricular fibrosis of spontaneously hypertensive rats (SHR).Methods Twenty SHR were randomly assigned to Chinese medicine group and model group.Additionally,ten Wistar-Kyoto(WKY) rats served as normal control group.After 12-week prevention,Masson staining method was used to measure the degree of fibrosis of left ventricular myocardial tissues,reverse transcription-polymerase chain reaction(RT-PCR) was used to detect Smad3 mRNA expression,and Western blotting method was used for the detection of Smad3 protein expression.Results The degree of left ventricular fibrosis myocardial tissue in Chinese medicine group was milder than that in the model group,and Samd3 protein and mRNA expression levels in Chinese medicine group were lower than those in the model group (P ＜ 0.05).Conclusion Kidney-reinforcing,blood-activating and phlegmresolving therapy can improve left ventricular fibrosis in SHR by inhibiting Smad3 expression.
