Metabolomic Study on Serum of Liver-Kidney Deficiency Syndrome of Knee-Joint Osteoarthritis Based on 1H-NMR
10.3969/j.issn.1005-5304.2017.06.007
- VernacularTitle:基于核磁共振氢谱的膝关节骨性关节炎肝肾亏虚证血清代谢组学研究
- Author:
Qin SHAO
;
Xiaoli ZHOU
;
Ying ZHANG
;
Bin WU
;
Shasha WANG
- Keywords:
metabolomics;
1H-NMR;
knee-joint osteoarthritis;
liver-kidney deficiency
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(6):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the endogenous metabolites of liver-kidney deficiency syndrome in knee-joint osteoarthritis (KOA) and explore the metabolic profile of KOA liver-kidney deficiency syndrome. Methods Totally 50 cases of KOA with liver-kidney deficiency syndrome and 50 cases of KOA with non-liver-kidney deficiency syndrome were collected respectively, and 20 cases of healthy volunteers were collected as the normal group. The serum samples of subjects were collected after fasting for 8 h. Hydro-Nuclear Magnetic Resonance (1H-NMR) spectrometer was collected. Principal component analysis and partial least squares discrimination analysis were used to conduct multivariate statistical analysis. Results 1H-NMR could identify 23 kinds of metabolites, and there was statistical significance between KOA patients and healthy volunteers (P<0.05, P<0.01). There was statistical significance in cartilage matrix metabolism, energy metabolism, lipid metabolism, pain, inflammation-related metabolites in patients with KOA liver-kidney deficiency syndrome and patients with KOA non-liver-kidney deficiency syndrome (P<0.05). Conclusion Patients with KOA liver-kidney deficiency syndrome have a unique 1H-NMR metabolic profile, KOA syndrome has a metabolic material basis.
