A correlational study of two methods of concentration determination for serum homocysteine: liquid chromatography-tandem mass spectrometry method and enzymatic cycling assay
10.13602/j.cnki.jcls.2017.03.03
- VernacularTitle:液相色谱串联质谱法和循环酶法测定血清中同型半胱氨酸的相关性
- Author:
Rui ZHAO
;
Pengfei LI
;
Weiyue YU
;
Ping DU
;
Zhixia ZHAO
;
Hongchuan LIU
;
Lihong LIU
- Keywords:
homocysteine;
LC-MS/MS method;
enzymatic cycling assay;
correlation
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(3):168-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the correlation between LC-MS/MS method and enzymatic cycling assay for determination of homocysteine concentration in human serum,and the application of two methods in the determination of homocysteine concentration.Methods Homocysteine concentrations of 63 serum samples were collected and determined by LC-MS/MS method and enzymatic cycling assay,respectively.The correlation between the concentrations by different methods was analyzed and evaluated.Results The concentrations were(19.11 ± 15.69) μmol/L by LC-MS/MS method and(16.95 ± 14.41) μmol/L by enzymatic cycling assay,the P value evaluated by paired-samples T test showed that there was statistical difference among the concentrations determined by two different methods (t =6.25,P ＜ 0.05).The conversion formula was YLC-MS/MS method =1.074Xenzymatic cycling assay + 0.892,R =0.987.Conclusion There is good correlation between LC-MS/MS method and enzymatic cycling assay for the determination of homocysteine concentration in serum,providing a theoretical basis for estimating the concentrations in the same serum sample by the two methods.
