Effects of Acupuncture on Oxidative Stress and Neuronal Damage in Prefrontal Cortex of Vascular Dementia Rats
10.3969/j.issn.1005-5304.2017.06.013
- VernacularTitle:针刺对血管性痴呆大鼠前额叶皮层氧化应激及神经元损伤的影响
- Author:
Siqi DU
;
Xuerui WANG
;
Wen ZHU
;
Caishuo JI
;
Tianran LI
;
Lingyong XIAO
;
Cunzhi LIU
- Keywords:
acupuncture;
vascular dementia;
oxidative stress;
neuronal damage;
SOD;
ROS;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(6):53-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of acupuncture on oxidative stress and neuronal damage in the prefrontal cortex of chronic cerebral hypoperfusion (CCH) rats; To investigate the molecular mechanisms of acupuncture in cognitive impairment of vascular dementia (VD) rats. Methods The animal model of VD was replicated by permanent bilateral common carotid artery occlusion (2VO) to induce CCH in rats. After modeling, rats were randomized into model group, acupuncture group and non-acupoint group. Sham-operation group was set as control group. Rats of acupuncture group and non-acupoint group were treated with needling for 2 weeks at 3 days after 2VO. Nissl and DHE staining were respectively used to evaluate the numbers of neurons and ROS level. SOD activity was examined by xanthine oxidase technique. Results Compared with sham-operation group, the number of neurons and SOD activity decreased in the model group, while the ROS level increased. Compared with the model group, the number of neurons and SOD activity increased in the acupuncture group, while the ROS level decreased. There were no obvious changes in all indexes in non-acupoint group. Conclusion Acupuncture can inhibit oxidative stress and improve neuronal damage and loss in the prefrontal cortex of VD rats.
