Detection and analysis of different genotypes of cervical cancer-associated miR-17-5p target loci
10.7683/xxyxyxb.2017.09.019
- VernacularTitle:宫颈癌相关miR-17-5p靶位点不同基因型检测分析
- Author:
Sanjun HE
;
Shaojun WEI
- Keywords:
cervical cancer;
miR-17-5 p;
single nucleotide polymorphism;
target site;
genotype
- From:Journal of Xinxiang Medical College
2017;34(9):851-853
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the different genotypes of cervical cancer-associated miR-17-5p target loci,and to analyze the influence of target site polymorphism on the risk of cervical cancer.Methods A total of 250 cervical cancer patients and 250 healthy females were selected in Hanzhong Central Hospital from June 2014 to June 2016.The blood samples were collected from the subjects.The genotypes of the three target loci rs3741216,rs217727 and rs2839702 in miR-17-5p were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The associativity between genetic polymorphisms and the risk of cervical cancer was calculated by SPSS 21.0 online software.Results The three candidate SNP loci fitted the Hardy-Weinberg equilibrium law.In the allele model,the rs217727 locus on the H19 gene significantly increased the risk of cervical cancer [OR =1.55,95％ CI(1.21,2.32),P =0.001].Genetic model analysis showed that rs217727 locus in the best model (dominant model),the risk of cervical cancer in the individuals carrying A/G and A/A genotypes increased significantly,and the risk of cervical cancer in the individuals carrying A/G and A/A genotypes was 1.65 times higher than that in the individuals carrying G/G genotype [OR =1.65,95％ CI (1.14,2.28),P =0.006].Conclusion The polymorphism of miR-17-5p target site rs217727 is associated with the risk of cervical cancer,the risk of cervical cancer in the individuals carrying A/G and A/A genotypes is significantly increased.
