Determination of perchlorate in food by ultra performance liquid chromatography-tandem mass spectrometry
10.13590/j.cjfh.2017.04.010
- VernacularTitle:超高效液相色谱-串联质谱法测定食品中高氯酸盐
- Author:
Weiwei HE
;
Jie YANG
;
Yuxin WANG
;
Yuzhe LI
;
Dawei CHEN
;
Yunfeng ZHAO
;
Shuang ZHOU
;
Congrong FANG
- Keywords:
Ultra performance liquid chromatography-tandem mass spectrometry;
perchlorate;
food contaminants;
food safety;
testing methods
- From:
Chinese Journal of Food Hygiene
2017;29(4):438-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of perchlorate in food by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods The perchlorate residue in spices and condiments was extracted with water,that in vegetables and fruits was extracted with acetonitrile-water (1∶ 1,V/V),and that in meat,poultry,eggs,milk and aquatic products was extracted with acetonitrile-water (2∶ 1,V/V).The supernatant was cleaned up with C18 SPE (3 ml,200 mg),and the detection was carried out by UPLC-MS/MS with internal standardmethod for quantification.Results The calibration curve was linear in the concentration range of 0.3-20.0 pg/L (R2 ≥0.999),the recovery was in the range of 82.6%-108.6%,the relative standard deviation (RSD) was in the range of 1.0%-9.9%,and the limit of detection was 2.0 μg/kg for milk,and 10.0 μg/kg for other food.Conclusion The method was simple,accurate and highly sensitive,and suitable for the determination of perchlorate in food.