Determination of chloramphenicol and metronidazolel in honey by ultra performance liquid chromatography-mass spectrometry with isotope-labelled internal standards
10.13590/j.cjfh.2017.04.012
- VernacularTitle:同位素内标-超高效液相色谱-串联质谱法测定蜂蜜中氯霉素及甲硝唑
- Author:
Ya TU
;
Jianping CUI
;
Hong ZHAO
- Keywords:
Ultra performance liquid chromatography-tandem mass spectrometry;
isotope labelled internal standards;
metronidazolel;
chloramphenicol;
honey;
food contaminants;
veterinary drug residues
- From:
Chinese Journal of Food Hygiene
2017;29(4):450-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine chloramphenicol and metronidazolel in honey by isotope-labelled internal standards ultra performance liquid chromatography-mass spectrometry.Methods Samples were extracted with ethyl acetate solution,and cleaned up on a MCS cartridge.The target analytes were separated on a ZORBAX SB-C18column with gradient elution using a mobile phase made up of methanol and 5 mmol/L ammonium acetate solution (containing 0.05％ formic acid).Detection was carried out using positive and negative electrospray ionization and multiple reaction monitoring (MRM),and quantified with isotope internal standardmethod.Results The chloramphenicol and metronidazolel showed good linearity in the range of 0.05-5.00 ng/ml.The recovery at three spiked levels of 0.5,2.0 and 5.0 μg/kg were in the range of 79.3％-96.7％.The relative standard deviation (RSD) was 5.5％-14.8％.The limits of quantitation were 0.15 μg/kg,the limits of detection were 0.05 μg/kg.Conclusion The method is sensitive and accurate.It could be applied to the high-throughput analysis of chloramphenicol and metronidazolel.
