3D Hydrogel Compression Model and Effect of Cyclic Compression on Osteoblast Differentiation
10.13523/j.cb.20170802
- VernacularTitle:3D水凝胶压应力模型建立及压应力对成骨细胞的影响
- Author:
Lingli ZHANG
;
Xiaoyang TONG
;
Jianmin GUO
;
Le LEI
;
Jun ZOU
- Keywords:
Compression;
Intensity;
Frequency;
Duration;
Osteoblasts-like cells
- From:
China Biotechnology
2017;37(8):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:3D hydrogel cell model was established,and cyclic compressive loading on MC3T3-E1 cell with different intensities,frequencies and durations was applied,in order to research the suitable solution about promoting the osteoblast differentiation with cyclic compression.Methods:Cyclic compressive loading on MC3T3-E1 cell was applied with different intensity,frequency and time.After compressive loading finished,the total RNA extraction from cell-gel constructs were performed and quantified ATF4,ALP,Runx2,Osteocalcin,RANKL and RANK mRNA.Results:RANKL and RANK mRNA expression significantly with different frequencies cyclic compressive loading (P < 0.05),and ALP mRNA (P < 0.05) and Runx2 mRNA (P < 0.01) expression significantly with different intensities and frequencies cyclic compressive loading (P < 0.05).Meanwhile,Runx2 mRNA expression with 4h significant higher than 12h (P < 0.05),and RANKL mRNA expression with 4h significant lower than 12h (P < 0.05).Conclusion:Determine the stress intensity and frequency,1% intensity,frequency of 0.5 Hz,4 h of cyclic compression intervention could promote the growth of osteoblasts-like cells in the 3D hydrogel model.