Construction and Characterization of Electrochemical Biosensor based on Endotoxin Aptameer
10.13523/j.cb.20170807
- VernacularTitle:细菌内毒素电化学生物传感器的构建及性能表征
- Author:
Yu YI
;
Minjun WANG
;
Jianfeng MEI
;
Jianshu CHEN
;
Yanlu ZHANG
;
Guoqing YING
- Keywords:
Aptamer;
Endotoxin;
Biosensor
- From:
China Biotechnology
2017;37(8):46-50
- CountryChina
- Language:Chinese
-
Abstract:
A biosensor was constructed to detect the endotoxin.The amine-terminated aptamer was immobilized on the gold electrode surface covered in advance with a self-assembled monolayer (SAM) of 3-mercaptopropionic acid (MPA).The modification of the gold electrode was confirmed by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).It was found that MPA assembly time at 6 h in the gold electrode surface to form a stable self-assembled monolayer.The biosensor has a good linear relationship with concentration of endotoxin in the range of 0.001 ～0.1 EU/ml,it is possible to be applied for quality control in the actual biological sample.
