Inhibitory effect of BEZ235 on human prostate carcinoma in vitro

VernacularTitle:BEZ235对体外前列腺癌细胞的抑制作用
Author: Guojun HOU ; Huilin QUE ; Jie SUN ; Tiefeng JIN
Keywords: BEZ235; prostate cancer; epithelial-mesenchymal transition
From: Journal of Central South University(Medical Sciences) 2017;42(8):869-873
CountryChina
Language:Chinese
Abstract: Objective:To determine effects of BEZ235,an inhibitor of phosphoionsitol-3-kinase (PI3K)/ mTOR,on the cell proliferation and migration in human prostate carcinoma lines including RWPE-1,PC3,and DU 145 cells.Methods:Viability of RWPE-1,PC3,and DU145 cells was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay,while cell migration was analyzed by wound healing assay.Western blot and immunofluorescence were used to examine the changes of relevant protein expression.Results:The proliferation of PC3 and DU145 cells was effectively inhibited by BEZ235 (P<0.01),whereas RWPE-1 was not obviously inhibited.Invasion and migration of PC3 and DU145 cells were attenuated by BEZ235 via EMT pathway.Conclusion:The PI3K/mTOR dual inhibitor BEZ235 shows substantial anti-tumor activity in human prostate carcinoma lines of PC3 and DU145 cells,which may be involved in the EMT pathway.