Effect of Shexiang Baoxin Pills on isoprenaline-induced myocardial cell hypertrophy and Cx43 expression
10.11817/j.issn.1672-7347.2017.08.006
- VernacularTitle:麝香保心丸对异丙肾上腺素诱导的心肌细胞肥大及其Cx43蛋白表达的影响
- Author:
Fen TANG
;
Zhentao JIANG
;
Wenting TAN
;
Junrong LONG
;
Shengquan LIU
;
Chun CHU
- Keywords:
Shexiang Baoxin Pill;
myocardial cell;
connexin 43;
myocardial hypertrophy;
gap junction
- From:
Journal of Central South University(Medical Sciences)
2017;42(8):906-910
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of Shexiang Baoxin Pill (SBP) on isoprenaline (Iso)-induced changes in myocardial cell volume,shape,and connexin 43 (Cx43) expression.Methods:HgC2 myocardial cells were randomly divided into a control group,a Iso group and a Iso+SBP group.After 72 h of culture,the average surface area of HgC2 cells was measured under phase contrast microscope.Bicinchoninic acid (BCA) protein assay was carried out to determine the concentration of proteins.The survival rate of myocardial cells was measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay,and the Cx43 expression was detected by Western blot.Results:The mean surface area and Cx43 concentration in Iso-treated myocardial cells were increased under the phase contrast microscope (P<0.05).Compared with the Iso group,the mean surface area was decreased,and the Cx43 concentration was reduced in the Iso+SBP group (both P<0.05).Compared with the control group,the Cx43 expression was obviously down-regulated in the H9C2 cells of the Iso group (P<0.05);while compared with the Iso group,the Cx43 expression was obviously up-regulated in the Iso+SBP group (P<0.05).Conclusion:Shexiang Baoxin Pills can prevent Iso-induced myocardial hypertrophy and down-regulate Cx43 expression.
