Detection of nucleic acid sequence-based amplification products by gold nanoprobe-based solution hybridization for the diagnosis of invasive aspergillosis
10.13602/j.cnki.jcls.2017.08.09
- VernacularTitle:纳米金探针液相杂交法检测核酸序列依赖扩增产物诊断侵袭性曲霉病
- Author:
Wenyao WU
;
Ruoyi HUA
;
Li DU
;
Qingquan PU
;
Jia YAN
;
Mi YANG
;
Yunyan HE
;
Yun XIA
- Keywords:
nucleic acid sequence-based amplification;
invasive aspergillosis;
Aspergillus;
gold nanoprobe
- From:
Chinese Journal of Clinical Laboratory Science
2017;35(8):593-596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of gold nanoprobe-based solution hybridization (GNBSH) to detect nucleic acid sequence-based amplification (NASBA) products for the rapid diagnosis of invasive aspergillosis (IA).Methods The Aspergillus specific 18S rRNA was amplified by NASBA and then the amplified products were hybridized with the gold nanoprobes which were modified with thiol compounds at the 5'end.Serum samples from 106 patients,including 14 with a definite IA,32 with suspected IA and 60 without IA,were detected by the established method,and the obtained results were compared with that of galactomannan (GM) test to evaluate its accuracy.Results The gold nanoprobes only hybridized with Aspergillus NASBA products but not other non-Aspergillus strains.The sensitivity,specificity and the area under the ROC curve (AUCROC) of the established GNBSH method for detecting 106 clinical samples were 82.61％ (38/46),81.67％ (49/60) and 0.890,respectively.The sensitivity,specificity and AUCROC of GM test were 56.52％ (26/46),83.33％ (50/60) and 0.723,respectively.Conclusion The established GNBSH method to detect Aspergillus NASBA products has high sensitivity and specificity and simple operation,which may be used to detect the infection of Aspergillus by clinical laboratories.
