Rapid determination of phosphodiesterase 5 inhibitors in invigorative health food by paper spray ionization mass spectrometry method
10.13590/j.cjfh.2017.04.014
- VernacularTitle:纸喷雾离子化质谱法快速筛查保健食品中违禁添加的磷酸二酯酶5抑制剂
- Author:
Ping WAN
;
Yuehua LU
;
Bo CHEN
- Keywords:
Paper spray ionization mass spectrometry;
rapid detection;
invigorative;
health food;
phosphodiesterase 5 inhibitors;
sildenafil;
vardenafil;
tadalafil;
adulterant
- From:
Chinese Journal of Food Hygiene
2017;29(4):459-463
- CountryChina
- Language:Chinese
-
Abstract:
Objective A rapid method was constructed for detection of 3 kinds of phosphodiesterase 5 (PDE5) inhibitors (sidenafil,vardalafil,tadalafil) in invigorative health food by paper spray ionization mass spectrometry (PSI-MS method).Methods The characteristic ions of the PDE5 inhibitors could be used for preliminary identification and semiquantitative analysis with internal standard method using PSI-MS method.Results The 24 kinds of commercially available health-care products includes capsule,tablet,pill,powder,wine,syrup and liquid were tested.Results from PSI-MS were consistent with the HPLC-UV date.The calibration curves of PSI-MS has a good linearity in a given range.The linear coefficients of analytes were higher than 0.99.The LODs of 3 kinds of PDE5 inhibitors were lower than 1.0 mg/L.The RSDs of this method ranged from 20％ to 24％.Conclusion The PSI-MS method was rapid,accurate and targeted,which is compliant for quickly screening the PDE5 inhibitors in large complex matrix samples.
