The study on nucleic acid testing for bacterial detection and its preliminary application on concentrated platelet
10.13303/j.cjbt.issn.1004-549x.2017.07.016
- VernacularTitle:核酸扩增法检测细菌的方法研究及其在浓缩血小板制品细菌检测中的初步应用
- Author:
Yuning LIU
;
Xun WANG
;
Danna FEI
;
Xuzan HAO
;
Shaou FU
;
Yao JIA
- Keywords:
bacterial contamination;
concentrated platelet;
nucleic acid testing;
automatic testing
- From:
Chinese Journal of Blood Transfusion
2017;30(7):702-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a nucleic acid testing assay for bacterial detection with meeting automatic requirement,and use it preliminarily to detect bacterial contamination in concentrated platelet.Methods Optimizing the bacterial DNA extraction methods,evaluating the sensitivity and specificity of the optimized assay with different bacterial strains,and applying the assay on bacterial detection for 100 units of concentrated platelet.Results The optimized extraction methods met the criteria of automatic testing.The sensitivity of the assay is 10,150,65,15 CFU/mL for Escheriehia coli,Stphylococcus aureus,Bacillus subtilis,and Pseudomonas aeruginas respectively.There was no non-specific amplification detected.And the assay could detect 1 contaminated bacterial from 100 units of concentrated platelet as same as BactALT bacterial culture.Conclusions Nucleic acid testing for bacterial genome 16S DNA could detect common contaminated bacterial sensitively and fast from platelet product.This assay could have the same effect when compared to bacterial culture methods when it was applied in concentrated platelet products.
