Study on theimmediate separation feasibility of PLT preparation by adding to carboxylation chitosan
10.13303/j.cjbt.issn.1004-549x.2017.07.059
- VernacularTitle:添加羧化壳聚糖采血后即刻分离制备血小板可行性研究
- Author:
Jiandi LIU
;
Tao ZHANG
;
Wei KANG
- Keywords:
blood component separation;
separation PLT preparation;
chitosan;
chitin
- From:
Chinese Journal of Blood Transfusion
2017;30(7):815-818
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the use of chitin derivatives-carboxylation chitosan immediate separation feasibility of PLT preparation,and to look for a new direction in separation feasibility of blood components.Methods 40 samples of blood donors were divided into the experimental group,natural sedimentation control group and centrifugal control group randomLy in Dalian.2 mL of whole blood were mixed with different concentrations of carboxylation chitosan which were diluted by blood preservation solution Ⅱ by according to the ratio of 1 ∶4.plasma precipitation amount were surveyed after 4 hours,with numbers of red blood cells,white blood cell and platelet,PLT aggregation and the changes of red blood cell morphology were observed.Finally,suitable amount of MAP were added into the optimal chitosan in preservation,and hemolysis of red blood cells were tested in 35 days.Results Suitable amount of chitin experimental group blood sedimentation rate were significantly faster than static device control group,and plasma remaining trace red blood cells,PLT-rich,and 35 days no obvious hemolysis.Conclusion carboxylation chitosan could be used in PLT preparation.
