Comparison on SDS-PAGE Gel Electrophoresis of Anti-liver Fibrosis Components in Pre- and Post-pro-cessed Trionyx Sinensis Carapace
10.3969/j.issn.1008-049X.2017.09.007
- VernacularTitle:鳖甲炮制前后抗肝纤维化活性部位的SDS-PAGE凝胶电泳比较研究
- Author:
Jianrong GAO
;
Zuliang HU
;
Jingni SHI
;
Yinping TANG
;
Yanwen LIU
;
Chunling HU
- Keywords:
Trionyx sinensis carapace;
SDS-PAGE gel electrophoresis;
Active components
- From:
China Pharmacist
2017;20(9):1543-1544,1563
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the difference in anti-hepatic fiborsis components between pre-and post-processed Trionyx Sinensis Carapace to guide the clinical application of Trionyx Sinensis Carapace. Methods:SDS-PAGE gel electrophoresis was used to compare the constituents in pre-and post-processed Trionyx Sinensis Carapace, and the inhibitory effect on the proliferation of HSC-T6 was deter-mined by MTT. Results:The processed Trionyx Sinensis Carapace had much more components than the crude Trionyx Sinensis Cara-pace,and the relative molecular mass in the first-level band decreased resulting in the generation of micromolecular polypeptides. Both pre-and post-processed Trionyx Sinensis Carapace had anti-hepatic fibrosis, while the vinegar-processed Trionyx Sinensis was more ef-fective. Conclusion:The difference in the active components in pre- and post-processed Trionyx Sinensis Carapace is obvious, which provides foundation for the clinical application and further researches.
