Establishment of HPLC Fingerprint and Classification of Chemical Components for Compound Sanhuang Tincture
10.3969/j.issn.1008-049X.2017.09.012
- VernacularTitle:复方三黄酊HPLC指纹图谱的建立和化学成分的归属
- Author:
Changling DING
;
Junfeng CUI
;
Xuemei ZHANG
;
Yong FU
;
Qizhong FAN
;
Wenna CHENG
- Keywords:
Anti-infection drugs;
Compound Sanhuang tincture;
HPLC;
Fingerprint;
Chemical component classification
- From:
China Pharmacist
2017;20(9):1564-1566,1572
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint of ethanol parts of compound Sanhuang tincture ( an anti-infective drug) using high performance liquid chromatography, and analyze the classification of the chemical components. Methods: A Phenomenex Luna C18 (2) (250 mm × 4. 6 mm, 5 μm) column was used. The mobile phase was acetonitrile-0. 05 mol·L-1 aqueous potassium di-hydrogen phosphate solution (22 :78) and the flow rate was 1. 0 ml·min-1 . The DAD detector was used and the detection wavelength was 237 nm. The column temperature was 30 ℃ and the injection volume was 10 μl. Results:The fingerprints of compound Sanhuang tincture were obtained with promising separation degree and the number of theoretical plates. A total of six fingerprint characteristic peaks were identified, and the reproducibility, stability and precision of the method were good. Meanwhile, combined with the informa-tion of retention time of compound medicine, single drug and reference substance, the source of characteristic peak of the effective part of compound Sanhuang tincture was determined. Conclusion:The fingerprints of compound Sanhuang tincture have strong characteris-tics and good reproducibility, which have important reference value for the quality evaluation of ethanol parts of compound Sanhuang tincture.
