Determination of Palmatine Hydrochloride and Berberine Hydrochloride in Baishi Shuitiao Powder by HPLC
10.3969/j.issn.1008-049X.2017.09.041
- VernacularTitle:HPLC法测定柏石水调散中盐酸巴马汀和盐酸小檗碱的含量
- Author:
Chuntong LI
;
Xiang LI
;
Jianli MA
;
Meifang ZHANG
- Keywords:
Baishi Shuitiao Powder;
Palmatine hydrochloride;
Berberine hydrochloride;
HPLC;
Content determination
- From:
China Pharmacist
2017;20(9):1668-1670
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the determination of palmatine hydrochloride and berberine hydrochloride in Baishi Shuitiao Powder simultaneously. Methods:A reversed phase ion pair HPLC method was adopted. An Agilent Zorbax SB-C18 (150 mm × 4. 6 mm,5 μm)column was used, the mobile phase consisted of acetonitrile-0. 1％ phosphoric acid (50 :50) (0. 2 g do-decyl sodium sulfate added to 100 ml solution), the flow rate was 1. 0 ml·min-1, the detection wavelength was at 345 nm, the injec-tion volume was 10 μl, the column temperature was at room temperature. Results: A good linear correlation was observed within the range of 1. 11-22. 16μg·ml-1 for palmatine hydrochloride (r=0. 9996) and 2. 11-42. 24μg·ml-1 for berberine hydrochloride (r=0. 9998). The average recovery was 98. 94％ with RSD of 2. 43％ for palmatine hydrochloride and 101. 3％ with RSD of 2. 05％ for berberine hydrochloride (n=6). Conclusion:The method is rapid, simple and accurate, and can be used for the content determina-tion of palmatine hydrochloride and berberine hydrochloride in Baishi Shuitiao Powder.
