Simultaneous Determination of Three Effective Components in Xinning Tablets by HPLC
10.3969/j.issn.1008-049X.2017.09.046
- VernacularTitle:HPLC法同时测定心宁片中3个有效成分的含量
- Author:
Xiaoying GUAN
;
Lingling JIANG
;
Shuhong WANG
;
Tiejie WANG
- Keywords:
HPLC;
Xinning tablets;
Rutin;
Salvianolic acid B;
Ginsenoside Rb1;
Quality control
- From:
China Pharmacist
2017;20(9):1681-1683
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an HPLC method for the simultaneous determination of rutin, salvianolic acid B and ginsen-oside Rb1 in Xinning tablets. Methods:The samples were separated on a CAPCELL PAK MG(250 mm × 4. 6 mm,5μm)column with gradient elution using acetonitrile(A) and 0. 1％ phosphoric acid(B) as the mobile phase at a flow rate of 1. 0 ml·min-1. The col-umn temperature was set at 35℃. The wavelengths were set on 354nm, 286nm and 203 nm (in a wavelength switching mode). Re-sults:The linear range of rutin was 0. 0273-1. 3600 mg·ml-1(r =1. 0000), that of salvianolic acid B was 0. 0244-1. 2200 mg· ml-1(r=1.0000),and that of ginsenoside Rb1 was 0.0186-0.9310 mg·ml-1(r=0.9999), and the average recovery (n=6) was 102.3％ (RSD=1.1％),98.7％ (RSD=0.8％) and 101.7％ (RSD=1.8％)(n=6), respectively. Conclusion: A rapid, simple, accurate HPLC method is successfully established for the simultaneous determination of 3 effective components in Xinning tab-lets, which is helpful to the quality control of Xinning tablets.
