Simultaneous Determination of Two Components in Furazolidone, Indometacin and Cuscohygrinolis α-Acetylbenzoacetate Suppositories by HPLC with Dual Wavelength Detection
10.3969/j.issn.1008-049X.2017.09.047
- VernacularTitle:HPLC双波长法同时测定美辛唑酮红古豆醇酯栓中的2种有效成分
- Author:
Yi SONG
;
Fanjian KONG
;
Yinke LI
;
Quan WANG
- Keywords:
HPLC;
Dual wavelength detection;
Furazolidone;
indometacin and cuscohygrinolis α-acetylbenzoacetate supposito-ries;
Furazolidone;
Indometacin
- From:
China Pharmacist
2017;20(9):1684-1686
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an analytical method for the determination of furazolidone and indometacin in furazolidone, in-dometacin and cuscohygrinolis α-acetylbenzoacetate suppositories by HPLC. Methods: The separation was performed on a ZORBAX Extend-C18 (250 mm × 4. 6 mm,5μm) column. The mobile phase was acetonitrile and 0. 035 mol·L-1 potassium phosphate monobas-ic aqueous solution (adjusting pH to 3. 0 with acetic acid) with gradient elution. The flow rate was 1. 0 ml·min-1, and the detection wavelengths were set at 364 nm and 318 nm. The column temperature was 30℃ and the injection volume was 20 μl. Results:Under the selected chromatographic conditions, the two components showed good linearity within the range of 0.005-0.05 mg·ml-1(r=0. 9999). The limit of detection was 20 ng·ml-1 and 26 ng·ml-1, respectively. The limit of quantitation was 70 ng·ml-1 and 90 ng·ml-1, respectively. The average recovery was 99. 4％(RSD=0. 6％, n=9)and 99. 4％(RSD=0. 3％,n=9),respectively. Conclusion:The method is simple, rapid and specific, and the results are accurate and reliable. The method can be used for the de-termination of the two components in furazolidone, indometacin and cuscohygrinolis α-acetylbenzoacetate suppositories.
