The Roles of Reactive Oxygen Species Produced by Contact Allergens and Irritants in Monocyte-derived Dendritic Cells.
- Author:
Dashlkhumbe BYAMBA
1
;
Tae Gyun KIM
;
Dong Hyun KIM
;
Jeong Hwan JE
;
Min Geol LEE
Author Information
- Publication Type:Original Article
- Keywords: Contact allergen; Contact dermatitis; Monocyte-derived dendritic cell; Reactive oxygen species
- MeSH: Allergens; Animals; Benzalkonium Compounds; Blotting, Western; Cysteine; Dendritic Cells; Dermatitis, Contact; Dinitrochlorobenzene; Flow Cytometry; HLA-DR Antigens; Humans; Irritants; Mice; Protein Carbonylation; Reactive Oxygen Species; Thimerosal
- From:Annals of Dermatology 2010;22(3):269-278
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Although reactive oxygen species (ROS) have been produced in both mouse bone marrow-derived dendritic cells (DCs) and XS-106 DCs by contact sensitizers and irritants in previous studies, the generation of ROS in human monocyte-derived DCs (MoDCs) and their role in contact hypersensitivity (CHS) has yet to be elucidated. OBJECTIVE: The purpose of this study was to determine whether contact allergens and irritants induce ROS in MoDCs and, if so, to evaluate the role of contact allergen and irritant induced-ROS in MoDCs in CHS. METHODS: Production of ROS was measured by 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) assay. Surface CD86 and HLA-DR molecules were detected by flow cytometry. Protein carbonylation was detected by Western blotting. RESULTS: ROS were produced by contact allergens such as dinitrochlorobenzene (DNCB) and thimerosal and the irritant benzalkonium chloride (BKC). DNCB-induced, but not BKC-induced, ROS increased surface CD86 and HLA-DR molecules on MoDCs and induced protein carbonylation. These changes were reduced in the presence of antioxidant N-acetyl cysteine. CONCLUSION: Our results suggest that DNCB-induced ROS may be different from those induced by irritant BKC. The DNCB-induced ROS may be associated with the CHS response, because they activate surface molecules on DCs that are important for generating immune reactions.
