Effects of Modified Zhisou Power on Epidermal Growth Factor Receptor and Neutrophil Elastase in Rats with Cold-Dryness Chronic Obstructive Pulmonary Disease
10.3969/j.issn.1005-5304.2017.10.013
- VernacularTitle:止嗽散加减方对慢性阻塞性肺疾病寒燥模型大鼠表皮生长因子受体及中性粒细胞弹性蛋白酶的影响
- Author:
Zhen GAO
;
Jing WANG
;
Min JIANG
;
Zheng LI
;
Dan XU
;
Upur HALMURAT
;
Jing JING
;
Fengsen LI
- Keywords:
COPD;
cold-dryness;
modified Zhisou Power;
epithelial growth factor receptor;
neutrophil elastase
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(10):53-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reveal the effects of cold-dryness and modified Zhisou Power on epidermal growth factor receptor (EGFR) and neutrophil elastase (NE) in rats with COPD. Methods COPD model was established with an elastase dose into the trachea combined with exposure to smoking for 90 d; cold-dryness COPD group was further developed by exposure to a cold, dry environment for 90 d. After 90 days, cold-dryness COPD rats was divided into cold-dryness group and Zhisou Power intervention group (treated with modified Zhisou Power for 7 days). On the 97th day, all rats were killed. Pathological changes in lungs were observed. mRNA and protein expression of EGFR were measured by RT-qPCR and Western blot, and the amount of NE in serum and BALF were examined by ELISA. Results EGFR mRNA and protein expression were significantly higher in COPD group, cold-dryness group and Zhisou Power intervention group than in control group. EGFR was significantly lower in Zhisou Power intervention group than in COPD group and cold-dryness group. NE was significantly higher in serum and BALF in COPD group, cold-dryness group and Zhisou Power intervention group than in control group. NE in BALF was significantly higher in cold-dryness group than in COPD group. NE in BALF was significantly lower in Zhisou Power intervention group than in cold-dryness group. Conclusion Modified Zhisou Power can down-regulate the expression of EGFR and the mount of NE in cold-dryness COPD rats to treat COPD.
