Evaluation of the value of anaerobic culture in bacterial contamination forapheresis platelets
10.13303/j.cjbt.issn.1004-549x.2017.07.063
- VernacularTitle:厌氧培养在单采血小板细菌筛查中的作用
- Author:
Liwei ZHU
;
Jian XU
- Keywords:
platelet contamination;
sterility testing;
bacterial detection;
anaerobic culture
- From:
Chinese Journal of Blood Transfusion
2017;30(7):826-828
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the potential deficiency due to lack of anaerobic culture and evaluate the effect to reduce adverse reaction associated to transfusion-translated bacterial infection.Methods The result of 9 758 units of apheresis platelet concentrates (PCs)detected with automated microbial detection system were reviewed and the medical records of the patients that received the contaminated PCs were followed.Results The confirmed positive rates by aerobic and anaerobic cultures were 0.06％ (6/9 758)and 0.16％ (16/9 758),respectively.In 10 of 16 yield cases,only the anaerobic culture was positive.The most of the bacterial detected by anaerobic culture only were Propionibacterium acnes.Their mean detection time from inoculation was 96.8±18.21 hours.Conclusion Addition of anaerobic culture would enhance the detection of bacterial contamination in PCs.However,since only slow-growing bacteria were detected,and because their clinical significance was debatable,blood service should select feasible and costeffective projects using only aerobic bottle for bacterial screening,like the majority of licensed blood centers in North America and Hong Kong,China.
