Determination of Three Bacteriostatic Agents in Taurine Eye Drops by HPLC
10.3969/j.issn.1008-049X.2017.09.052
- VernacularTitle:HPLC法测定牛磺酸滴眼液中3种抑菌剂含量
- Author:
Ying YANG
;
Rui MAO
;
Naijiang CHEN
- Keywords:
Taurine eye drops;
Bacteriostatic agents;
Content;
HPLC
- From:
China Pharmacist
2017;20(9):1697-1699
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for the determination of three bacteriostatic agents ( methylparaben, eth-ylparaben, propylparaben) in taurine eye drops. Methods:The HPLC method was conducted on a Phenomenex Gemini C18 column ( 250 mm × 4. 6 mm, 5 μm ). The mobile phase was 1％ acetic acid-methanol(40 :60). The flow rate was 1. 0 ml·min-1 and the de-tection wavelength was 254 nm with the injection volume of 20 μl and the column temperature of 25℃. Results:Methylparaben, eth-ylparaben and propylparaben showed good linear relationship (r>0. 999)within the range of 1. 00-19. 94μg·ml-1,2. 01-20. 08μg· ml-1 and 0. 21-10. 46 μg·ml-1,respectively. The average recoveries were 99. 20％-99. 90％, and the RSDs were 1. 34％-1. 54％(n=9). Conclusion:The method is accurate, reproducible and stable without interference, which can be used for the determination of three bacteriostatic agents in taurine eye drops.
