Simultaneous Determination of Paeoniflorin, Tetrahydropalmatine and Osthole in Gujinwan Capsules by HPLC

VernacularTitle:HPLC法同时测定骨筋丸胶囊中芍药苷、延胡索乙素和蛇床子素的含量
Author: Qingzheng JIN ; Ming ZHENG
Keywords: Gujinwan capsules; Paeoniflorin; Tetrahydropalmatine; Osthole; HPLC
From: China Pharmacist 2017;20(9):1694-1696
CountryChina
Language:Chinese
Abstract: Objective:To develop an HPLC method for the content determination of paeoniflorin, tetrahydropalmatine and osthole in Gujinwan capsules. Methods:The determination was performed on an Eclipse XDB-C18 column (250 mm × 4. 6 mm,5 μm) with the mobile phase consisting of 0. 1％ phosphoric acid (adjusting pH to 6. 6 with triethylamine)-acetonitrile with gradient elution. The flow rate was 1. 0 ml·min-1 . The column temperature was 30 ℃. The detection wavelengths were set at 230 nm and 280 nm. Re-sults:The calibration curves were found to be linear within the range of 9. 56-33. 65 μg· ml-1 for paeoniflorin(r=0. 9998), 3. 65-12. 86 μg· ml-1for tetrahydropalmatine(r=0. 9999) and 5. 81-20. 45 μg· ml-1for osthole(r=1. 0000). The average recovery of paeoniflorin, tetrahydropalmatine and osthole was 98. 8％(RSD=1. 1％), 98. 4％(RSD=0. 8％) and 99. 1％(RSD=1. 4％)(n=6),respectively. Conclusion:The method is simple, accurate and reproducible, which can be used for the quality control of Gujinwan capsules.