Extraction Optimization of Anthraquinones in Semen Cassiae by Box-Behnken Experimental Design
10.3969/j.issn.1008-049X.2017.09.010
- VernacularTitle:Box-Behnken效应面法优化决明子中蒽醌类成分提取工艺
- Author:
Guanghui FU
;
Jianfeng ZHAN
- Keywords:
Semen Cassiae;
Chrysophanol;
Aurantio-obtusin;
Extraction technology;
Box-Behnken experimental design
- From:
China Pharmacist
2017;20(9):1554-1558
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction parameters for anthraquinones in Semen Cassiae by Box-Behnken experimental design. Methods:Based on single factor screening, a three-factor and three-level Box-Behnken experimental design was employed with the ethanol concentration, material to liquid ratio and extraction time as the independent variables. The dependent variables including the extration rates of chrysophand and aurantio-obtusin were transformed into desirability mathematically by Hassan 's method. The mathematical relationship was established between the dependent variables and the independent variables. The optimum experimental conditions were selected from the stationary point of the response surfaces. Results:The optimum extraction conditions were as follows:12-fold amount of 44％ ethanol as the solvent, extracted three times with 2. 65h for each time. The extraction rate of chrysophanol and aurantio-obtusin was 1. 921％ and 3. 244％, respectively. Conclusion:The measured value is close to the predicted one,which indi-cates the comprehensive extraction parameters optimized by Box-Behnken experimental design can be used for the extraction of chry-sophanol and aurantio-obtusin from Semen Cassiae.
