Effect of Hydroxysafflor Yellow A on Pro/Anti-Inflammatory Cytokines in Peripheral Blood with Sepsis in Mice
- VernacularTitle:羟基红花黄色素A对脓毒症小鼠外周血促炎/抗炎因子的影响
- Author:
Jinping WANG
;
Ping WANG
;
Runhua CHEN
;
Peiyan ZHAO
;
Zheyu HUANG
;
Hanwei WU
;
Jianlong WU
- Keywords:
sepsis;
hydroxysafflor yellow a;
inflammatory cytokine;
bacterial load
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(5):665-669
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore the effect of HSYA on pro/anti-inflammatory cytokine′s levels and mRNA expression in peripheral blood of mice.with sepsis.[Methods]Dividing NIH mice into four groups,as normal group,sham group,CLP group and HSYA group,24 mice in each group. The CLP sepsis mouse model was established. HSYA(120 mg/kg)were injected intravenously at 12 h before the operation ,and 0 h and 12 h following CLP ,and other groups were given normal saline. observed the animals behavior changes,measured the levels WBC,PLT,ALT,AST,BUN in serum,detected the levels and mRNA expression of IL-6, IL-10 ,TNF-α in peripheral blood. cultured of peripheral blood bacteria loads.[Results]24 h after surgery ,mice in CLP group appeared furring,feces residues on anus etc. compared to normal group,sham group and HSYA group,WBC,PLT,ALT,AST, BUN,levels and mRNA expression of IL-6,IL-10,TNF-αshowed significant increases,it was also found that bacterial load was significant increased in model group.[Conclusions]HSYA has a therapeutic effect in mice with sepsis ,can reduce bacteria into the blood,and inhibit inflammatory mediators which caused tissue damage.
