Simultaneous Determination of Monocaffeyltartaric Acid, Chlorogenic Acid, Caffeic Acid and Chicoric Acid in Herba Taraxaci by HPLC
10.3969/j.issn.1008-049X.2017.09.044
- VernacularTitle:HPLC法同时测定蒲公英中单咖啡酰酒石酸、绿原酸、咖啡酸、菊苣酸的含量
- Author:
Yan LIU
;
Ruirong LAN
;
Jingdong DUI
;
Ying SU
- Keywords:
HPLC;
Herba Taraxaci;
Monocaffeyltartaric acid;
Chlorogenic acid;
Caffeic acid;
Chicoric acid;
Content determina-tion
- From:
China Pharmacist
2017;20(9):1677-1679
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an HPLC method for the simultaneous determination of four phenolic compounds ( monocaffey-ltartaric acid, chlorogenic acid, caffeic acid and chicoric acid) in Herba Taraxaci. Methods:The separation was performed on a Ther-mo C18 column (250 mm × 4. 6 mm, 5 μm) with methanol-0. 2％ phosphonic acid as the mobile phase with gradient elution. The de-tection wavelength was 328 nm, the flow rate was 1. 0 ml·min-1 and the column temperature was 35℃. Results:The linear range of monocaffeyltartaric acid, chlorogenic acid, caffeic acid and chicoric acid was 11.59-115.90 (r = 0.9999), 2.50-24.96(r =0. 9998),2. 27-22. 70(r=0. 9995) and 12. 44-124. 40(r=0. 9998) μg ·ml-1, respectively. The average recovery was 102. 50％(RSD=2. 30％), 99. 29％(RSD=0. 43％), 96. 71％(RSD=0. 78％) and 95. 36％ (RSD=1. 30％), respectively. Conclusion:The method is simple and accurate with good repeatability, which can be used for the quality control of Herba Taraxaci.
