Effects of panax notoginseng saponins on expression of IL-10 and TNF-α in rats with focal cerebral ischemia-reperfusion
10.11659/jjssx.05E017032
- VernacularTitle:三七总皂苷对大鼠局灶性脑缺血再灌注后IL-10及TNF-α表达的影响
- Author:
Xiaoming DING
;
Rong XU
- Keywords:
panax notoginseng saponins;
cerebral ischemia;
reperfusion injury;
IL-10;
TNF-α
- From:
Journal of Regional Anatomy and Operative Surgery
2017;26(9):634-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of panax notoginseng saponins(PNS) on the expression of IL-10 and TNF-α in the brain after focal cerebral ischemia-reperfusion injury in rats,and the mechanism was explored.Methods 30 male adult Sprague-Dawley rats were randomly divided into sham-operation group,ischemia-reperfusion group and PNS treatment group,10 rats in each group.The middle cerebral artery occlusion(MCAO) was established with ischemia-reperfusion group.The rats received continuous gastric lavage for 4 weeks,who were observed for 3 days and then killed to the detect the expression of IL-10 and TNF-αin ischemic region by ELISA,immunohistochemistry and RT-PCR.The behavioral ability score in rats were tested.Results Compared with the sham-operation group and ischemia-reperfusion group,the expression of IL-10 was increased,while the expression of TNF-α was decreased significantly in PNS treatment group,behavior score of PNS treatment group increased significantly than that of ischemia-reperfusion group.Conclusion The panax notoginseng saponins can reduce the neurons injury after focal cerebral ischemia-reperfusion injury in rats partly by regulating the expression of IL-10 and TNF-α in the brain.
