Role of Wnt/β-catenin signaling pathway in migration of asthmatic rat airway smooth muscle cells
10.11659/jjssx.01E017068
- VernacularTitle:Wnt/β-catenin信号通路对哮喘大鼠气道平滑肌细胞迁移能力的影响
- Author:
Yong ZHANG
;
Xiaofen WANG
- Keywords:
Wnt/β-catenin;
asthma;
asthmatic;
airway smooth muscle cells;
migration
- From:
Journal of Regional Anatomy and Operative Surgery
2017;26(9):643-647
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of Wnt/β-catenin signaling pathway in migration ability of asthmatic rat airway smooth muscle cells (ASMCs).Methods The asthmatic rat airway smooth muscle cells were cultured in vitro.The expression of Wnt/β-catenin in ASMCs was detected by RT-PCR and Western blot.Migration ability of ASMCs was detected by wound healing assay and transwell migration assay.Results Compared with normal rats,the expression level of Wnt/β-catenin in chronic asthmatic rats ASMCs was obvious higher.The β-catenin signaling pathway by specific siRNA could reduce beta-catenin mRNA and protein expression level of ASMCs.The knockdown of Wnt/β-catenin was able to decreases the migration ability of ASMCs.Conclusion Abnormal activation of Wnt/β-catenin signaling pathway may participants in the migration ability of ASMCs of chronic asthma rats.
