Expression of PRR11 and its effect on proliferation of human osteosarcoma cells
10.11659/jjssx.03E017064
- VernacularTitle:骨肉瘤中PRR11蛋白的表达及对骨肉瘤进展的影响
- Author:
Xiaojun ZHANG
;
Yong XI
;
Peng FENG
;
Jie LI
;
Da LIU
- Keywords:
PRR11;
osteosarcoma;
proliferation;
metastasis;
progression
- From:
Journal of Regional Anatomy and Operative Surgery
2017;26(9):625-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of PRR11(Proline-rich protein 11) in human osteosarcoma and investigate the effect of PRR11 on the proliferation of human osteosarcoma cells.Methods Immunohistochemical staining was applied to detect the PRR11 expression in 75 cases of osteosarcoma and corresponding normal tissues.Western blotting was used to examine PRR11 protein expression levels in osteosarcoma cell lines.We used siRNA to knock down the expression of PRR11 and tested the effects of PRR11 down-regulation on the proliferation in SaOS2 cells.Results PRR11 was overexpressed in osteosarcoma specimens compared to their paired normal tissues,the over expression rate of PRR11 in osteosarcoma and corresponding paracancerous tissues were 76％(57/75) and 9.33％(7/75) with statistical difference(P<0.05).The high expression of PRR11 was correlated with tumor pathological grade and lymphatic metastasis(P<0.05).PRR11 was expressed in 4 osteosarcoma cell lines which were SaOS2,143B,U2OS and MG63 respectively,the expression was highest in SaOS2 cells.Silencing PRR11 inhibited cell growth as compared with control cells(P<0.05).Conclusion PRR11 is overexpression in human osteosarcoma and promotes its progression by enhancing proliferation.The increased expression of PRR11 in osteosarcoma is a new target for treatment and early diagnosis of human osteosarcoma patients.
