The effect of interleukin-17 on podocyte-associated factors and apoptosis and molecular mechanism
10.3760/cma.j.issn.1673-4912.2017.08.006
- VernacularTitle:白细胞介素-17对足细胞相关分子和凋亡的影响及其分子机制研究
- Author:
Li WANG
;
Qiu LI
;
Lijia WANG
;
Haiping YANG
;
Cuicui LI
;
Shipin FENG
- Keywords:
Interleukin-17;
Podocyte;
Apoptosis;
Fas;
Podocylaxin
- From:
Chinese Pediatric Emergency Medicine
2017;24(8):587-592
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of interleukin(IL)-17 on podocyte-associated factors and apoptosis in podocytes and explore the molecular mechanism.Methods The podocytes were used as the object of study.The apoptosis of podocytes induced by IL-17 in a dose（1nm/ml，10nm/ml，50nm/ml，100nm/ml） and time（12h，24h，48h，72h） way and the protein expression of Fas and FasL of podocytes induced by IL-17 were detected by flow cytometrey.The podocytes were divided into the blank control group and 100ng/ml IL-17 induction group.The expressions of mRNA of Nephrin，WT1，Synaptopodin，Podocylaxin，Fas and FasL were measured by Real-time RT PCR.The proteins of WT1，Caspases8，Caspases3 in podocyte were detected by immunocytochemistry method.Results IL-17 promoted the apopotosis of podocyte in a dose and time way（P<0.01），and increased the expression of Fas，Caspase 8 and Caspase 3 in podocyte（P<0.01）.IL-17 decreased the expression ofPodocylaxin （P<0.05），but had no effect on Nephrin，WT1，Synaptopodin，and FasL in podocyte（P＞0.05）.Conclusion IL-17 may causerenal injure by inducing the apoptosis of podocyte and decreasing the expression of Podocylaxin in podocyte.
