Dynamic changes of glutamate during cerebral ischemia in the cortex of cynomolgus monkeys
10.16016/j.1000-5404.201701086
- VernacularTitle:食蟹猴脑缺血过程中皮层谷氨酸水平动态变化的研究
- Author:
Chen WEI
;
Guoxian TAO
;
Rongping TANG
;
Guolong LIU
;
Zhiming ZHANG
;
Feng YUE
- Keywords:
brain ischemia;
glutamate acid;
middle cerebral artery;
cynomolgus monkeys
- From:Journal of Third Military Medical University
2017;39(17):1728-1733
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the dynamic changes of glutamate in the cortex of cynomolgus monkeys during cerebral ischemia.Methods Proximal M1 segment of middle cerebral artery (MCA) was occluded for 1 h in 3 young cynomolgus monkeys (7.3 ± 1.5 years old) to induce cerebral ischemia.Magnetic resonance imaging and neurologic deficit scoring were used to evaluate the ischemia and observe the manifestations,respectively.Fast Analytical Sensing Technology (FAST) was applied to record the content of cortex glutamate in the same site of ipsilateral primary motor cortex in the periods of pre-,during,and post-occlusion,and at 1 and 2 weeks after surgery.Results Compared with pre-occlusion,the content of glutamate was increased significantly in the process of occluding in the MCA M1 (P =0.003);No significant difference was observed in the content during occluding and post-occlusion (P--0.877).The content in the first week was decreased obviously as compared with post-occlusion (P--0.004),but it showed no statistical difference with that in the second week (P =0.085).Conclusion Cerebral ischemia may potentially accelerate the extra-cellular glutamate release in the cortex,but reperfusion may ameliorate or balance off the glutamate release.
