STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro
10.16016/j.1000-5404.201612168
- VernacularTitle:下调STYK1基因表达可抑制结直肠癌细胞生长与侵袭
- Author:
Jiajia TANG
;
Lin YE
- Keywords:
serine/threonine/tyrosine kinase 1;
colorectal cancer;
proliferation;
invasion
- From:Journal of Third Military Medical University
2017;39(17):1734-1738
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 (STYK1) on the proliferation,apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15.Methods The expression of STYK1 protein was downregulated by RNA interference technique in HCT-15 cells.Then,the STYK1 protein expression was detected by Western blotting.CCK-8 assay was used to measure the effect of STYK1 down-regulation on the proliferation of the cells,flow cytometry was employed to detect the cell apoptosis,and Transwell chamber assay was adopted for cell invasion ability.The expression levels of p-ERK1/2 and p-AKT were detected by Western blotting.Results Compared with the siRNA NC group,STYK1 down-regulation significantly inhibited the proliferation (P ＜ 0.01) and invasion (P ＜ 0.01),induced cell apoptosis (P ＜ 0.01),and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA (P ＜0.01).Conclusion STYK1 down-regulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells,which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.
