Research of Bone Marrow Mesenchymal Stem Cell Transplantation for Rat Cerebral Infarction Recovery of Neurological Function
10.13241/j.cnki.pmb.2017.22.006
- VernacularTitle:骨髓间充质干细胞移植对脑梗死大鼠神经功能恢复的影响
- Author:
Tao WANG
;
Xiaohong WANG
;
Suping WANG
;
Yunxia DU
;
Shufang ZHAO
;
Sijia WANG
- Keywords:
Bone marrow mesenchymal stem cells;
Transplantation;
Cerebral infarction
- From:
Progress in Modern Biomedicine
2017;17(22):4227-4231
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of BMSC transplantation on the recovery of neurological function in rats with cerebral infarction,and to explore the related mechanism.Methods:90 rats were randomly divided into 3 groups:sham operation group,control group,BMSC transplantation group,30 rats in each group.The control group and BMSC transplantation group established middle cerebral artery occlusion (MCAO) model,the sham operation group only need to separate the cervical tissue of rats,and MCAO model in the MCAO model operation.After 1 days of BMSC transplantation group by intravenous injection of 1 mL 3× 106 BMSC,the control group was injected with the same dose of NS in MCAO after 1 D,3 D,7 d,14 d,21 d,28 d,35 d,42 d,49 D respectively,the neurological function score of rats (mNSS),after 2 months of transplantation BMSC group and control group of brain tissue for immunohistochemical staining,detection of MAP2,TUJ1,Ⅷ factor,the expression of GFAP.Results:In seventh to thirty-fifth days after treatment,BMSC mNSS transplantation group were significantly lower than the control group (P ＜ 0.05).2 months after BMSC transplantation group MAP2,TUJ1,Ⅷ expression level was significantly higher than the control group,while the control group,the expression of GFAP was significantly higher than that of BMSC group (P ＜ 0.01).Conclusion:BMSC transplantation in order to promote the recovery of neurological function in cerebral infarction.
