Effects of Neuroligin on the Proliferation and Apoptosis of SH-SY5Y Cells
10.13241/j.cnki.pmb.2017.22.010
- VernacularTitle:Neuroligin对SH-SY5Y细胞增殖和凋亡的影响
- Author:
Yuzhi WU
;
Yanqin GUO
;
Chunlei YU
;
Xiaofan YANG
;
Pengxiang BI
;
Ning AN
;
Wenhui CAO
- Keywords:
Neuroloigin;
Neuron;
Apoptosis;
PSD-95;
Bax
- From:
Progress in Modern Biomedicine
2017;17(22):4246-4249
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study effects of neuroligin (NLG) on the proliferation and apoptosis in human neuroblastoma SH-SY5Y cells.Methods:The SH-SY5Y cells were cultured in vitro for 24 hours,and then transfected with NLG siRNA at dose of 50,100,200 μmol/L,respectively.MTT procedure was used to detect the cell proliferation,and expression levels of apoptosis gene including Bax or Bcl-2 and Bcl-xl were measured by RT-PCR.Results:Compared to control groups proliferation of SH-SY5Y cells were distinctly inhibited after NLG siRNA transfection accompany with a dose-dependent,which was caused by activation of apoptosis.Conclusions:NLG protect neuron by inhibiting apoptosis.
