Over-expression and suppression of HMGBI affect proliferation and invasion of endometrial carcinoma and underlying mechanisms
10.11817/j.issn.1672-7347.2017.07.005
- VernacularTitle:HMGBI过表达和沉默影响子宫内膜癌HEC-IA细胞增殖和侵袭及其机制
- Author:
Jing WANG
;
Yuqi GAO
;
Xinyi LIU
;
Jiajie WU
- Keywords:
high mobility group box-1 protein 1;
endometrial carcinoma;
over-expression;
suppression;
proliferation;
invasion
- From:
Journal of Central South University(Medical Sciences)
2017;42(7):769-775
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effects of over-expression and suppression of HMGB1 on proliferation and invasion of endometrial carcinoma HEC-1A cell and underlying mechanisms.Methods:Over-expression or silence of HMGB 1 in HEC-1A cell lines were established by lentiviral vector containing HMGB 1 recombinant plasmid or by HMGB 1 shRNA,respectively.Cell counting kit-8,Transwell,and wound healing assay were used to analyze proliferation,invasion,and migration of HEC-1A cells,respectively.Western blot and reverse transcription-PCR (RT-PCR) were used to detect the expression of NF-κB,VEGF,and matrix metalloproteinase 2 (MMP2) in the cells.Results:Over-expression of HMGB1 promoted the proliferation,invasion,and migration of HEC1A cell,and up-regulated NF-κB,VEGF,and MMP2 expressions,while suppression of HMGB1 inhibited the proliferation,invasion,and migration of HEC-1A cells and down-regulated NF-κB,VEGF,and MMP2 expressions.Conclusion:HMGB1 plays an important role in proliferation,invasion,and migration of HEC1A cell via NF-κB/VEGF/MMP2 pathway.HMGB 1 might be a potential target for endometrial carcinoma therapy.
