Development and evaluation of a rapid test strip for gB antibody detection of pseudorabies virus

VernacularTitle:猪伪狂犬病gB抗体快速检测试纸条的研制和评价
Author: Rongchao QIU ; Zhanbing HAN ; Yongxiang LIU ; Guopan TANG ; Wengang LI
Keywords: pseudorabies virus; strip; ELISA
From: Chinese Journal of Veterinary Science 2017;37(8):1463-1467
CountryChina
Language:Chinese
Abstract: To evaluate the gB antibody level of pseudorabies virus (PRV) in swine,a rapid test strip was developed.In the strip,the expressed protein of gB was labeled with colloidal gold,the staphylococcal protein A (SPA) and swine anti PRV antibody were blotted on the nitrocellulose membrane for the test and control lines,respectively.The specificity and sensitivity of the strip were detected with standard positive,negative and immunized sera of PRV,the results indicated that the strip was high specificity and sensitivity.Field swine serum samples were tested by the new strip and commercial IDEXX PRV gB ELISA kit,simultaneously.The agreement rate of the two methods was 91.04％.Furthermore,the dipstick assay based on the strip is rapid (5 min),sensitive and easy to perform.This suggests that the new strip is an acceptable alternative for field diagnosis.