Effect of Newcastle disease virus-like particles on dendritic cell maturation
10.16303/j.cnki.1005-4545.2017.08.09
- VernacularTitle:新城疫病毒样颗粒对树突状细胞成熟的影响
- Author:
Jiaxin DING
;
Jing QIAN
;
Xiaohong XU
;
Jindou LI
;
Lingsong QIN
;
Lei HUANG
;
Shuting WU
;
Wei DING
;
Renfu YIN
;
Zhuang DING
- Keywords:
Newcastle disease virus;
virus-like particles;
dendritic cells;
maturation;
innate immunity
- From:
Chinese Journal of Veterinary Science
2017;37(8):1485-1489,1576
- CountryChina
- Language:Chinese
-
Abstract:
Newcastle disease virus-like particles (NDV VLPs) are composed of matrix protein (M) as the skeleton,with the insertion of hemagglutinin-neuraminidase and/or fusion protein.NDV VLPs are reported to be immunogenic and can induce specific humoral and cellular immune responses.However,its relationship with innate immunity remains elusive.Dendritic cells (DCs) are a group of specialized antigen presenting cells,which are crucial in connecting innate immunity and adaptive immunity.In this study,NDV VLPs and murine DCs were used to investigate the connection between NDV VLPs and innate immunity.The DC maturation induced by NDV VLPs (M+ HN) was evaluated.The results showed that NDV VLPs could be effectively taken up by DC and presented to naive T cells.NDV VLPs-induced DC significantly up-regulated the expression of MHC Ⅱ and costimulatory molecules on DC surface,and subsequently promoted the secretion of proinflammatory cytokines.This experiments also showed that different assembled NDV VLPs induced significant stimulating ability in cytokine levels.In summary,NDV VLPs can induce DC maturation,which gives insights to better understanding of VLPs-mediated innate immunity and provide information in selecting preferred NDV VLPs candidate.
