Influence of the growth traits and virulence genes of Enterococcus faecalis isolate N41 by cocultivation with Salmonella
10.16303/j.cnki.1005-4545.2017.08.17
- VernacularTitle:共培养沙门菌对粪肠球菌N41株生长特性及毒力基因的影响
- Author:
Erli WU
;
Chunhao HE
;
Yue JIN
;
Lingling LIU
;
Hui HU
;
Han CAO
;
Yabin WANG
;
Liying CHEN
- Keywords:
Enterococcus faecalis N41;
Salmonella;
growth trait;
virulence gene;
real-time PCR
- From:
Chinese Journal of Veterinary Science
2017;37(8):1528-1533
- CountryChina
- Language:Chinese
-
Abstract:
To explore the impact of Salmonella on Enterococcusfaecalis isolate N41 under the coexisting,the growth traits and the transcription of 26 virulence genes of N41 at various growth phases were detected.Salmonella and/or N41 were inoculated and done plate counting,then growth curves were drawn and bacterial total RNA were extracted at given time points,quantitive realtime PCR was used to analyze the RNA transcription levels of 26 virulence genes of N41.The result showed that comparison with single incubation,the bacteria concentration of N41 dropped about 3.9 times,and Salmonella dropped about 110-times.Among 26 virulence genes of N41,the RNA transcription levels of 12 virulence genes such as ebpA,ebpC,rnjB,ace,ebpR,psr and so on were promoted at the four growth phases,but the RNA transcription levels of SlyA and sprE were dropped.Except that the RNA transcription levels of CylL-S,CylL-L,efaA and AS had no significant changes at the four growth phases,the mostly rest genes increased dramatically at post-log phase.This indicated that when incubated together,N41 inhibited the growth of Salmonella significantly,and Salmonella promoted the transcription levels of virulence genes of N41 as a whole.The results lay a foundation for further research on the interaction between bacteria species and the pathogenicity mechanism of Enterococci.
