Development of high-performance liquid chromatography methods for the anticoccidials: toltrazuril and diclazuril.
10.14405/kjvr.2017.57.4.223
- Author:
Kyung Hun JEONG
1
;
Miyoung JEONG
;
Hae Chul PARK
;
Md Akil HOSSAIN
;
Daegyun KIM
;
Kwang Jick LEE
;
Jeong Woo KANG
Author Information
1. Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea. hijach@korea.kr
- Publication Type:Original Article
- Keywords:
coccidiostats;
diclazuril;
high performance liquid chromatography;
toltrazuril
- MeSH:
Chromatography, High Pressure Liquid;
Chromatography, Liquid*;
Coccidiostats;
Methods*;
Sensitivity and Specificity;
Veterinary Drugs
- From:Korean Journal of Veterinary Research
2017;57(4):223-226
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was undertaken to develop new analytical methods for assessment of anticoccidials. High-performance liquid chromatography (HPLC) was found to be a fast, reliable, and practical method. The anticoccidials used in this experiment were toltrazuril and diclazuril, and the analysis factors were specificity, linearity, accuracy, repeatability, and intermediate precision. The linearity of each anticoccidial was better than 0.99, and the accuracies were 99.5% and 99.1% with relative SD of 0.5 and 0.4, respectively. To assess whether the developed HPLC method could be effectively applied, toltrazuril and diclazuril post-market veterinary products (five products) that are currently sold were tested. The results revealed no non-compliant items and the method was applied successfully. Therefore, the newly developed HPLC method for anticoccidial assessment described in this study may be useful as a reference method in the Korean Standards of Veterinary Pharmaceuticals for the analysis of toltrazuril and diclazuril.
