Predictive prognostic value of BRCA1 screened by whole genome expression profiling in ductal carcinoma in situ
10.3760/cma.j.issn.1673-4203.2016.12.006
- VernacularTitle:基于全基因组表达谱芯片筛查的BRCA1对乳腺导管内癌预后的预测价值
- Author:
Yongnan WANG
;
Mei YANG
;
Anqin ZHANG
;
Liang ZHANG
;
Wenping LI
;
Zhenqiang LIAN
;
Zhou HE
;
Huibin LI
- Keywords:
Ductal carcinoma in situ;
Gene expression profiling;
Comparative Genomic Hybridization;
BRCA1 protein;
Prognosis
- From:
International Journal of Surgery
2016;43(12):810-814,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the predictive prognostic value of BRCA1 screened by whole genome expression profiling in ductal carcinoma in situ.Methods Collected 4 cases of breast ductal carcinoma and 4 cases of breast invasive ductal carcinoma fresh samples from January 2014 to June 2014,and the difference of BRCA1 expression on whole genone expression profiling was analyzed by microarray comparative genomic hybridization.The expression of BRCA1 was detected by immunohistochemistry in 70 cases of ductal carcinoma in situ of the breast,and the prognosis of intraductal carcinoma was evaluated.Results BRCA1 gene differentially expressed in invasive ductal carcinoma and ductal carcinoma in situ by screening.The positive rate of BRCA1 protein in breast ductal carcinoma in 14.3％ (10/70),its expression had no significant relationship with age (P =0.959),menopause (P =0.959),tumor size (P =0.627),axillary lymph node status (P =1.000),HR status (P =0.958),HER-2 status (P =1.000),P53 expression (P =0.460).ductal carcinoma with micro-infiltration ratio in BRCA1 negative group was higher than BRCA1-positive group (P =0.043).The median follow-up of 47 months,Disease-free survival rate of all was 97.1％.Disease-free survival of BRCA1 negative group and BRCA1-positive group had no significant difference (96.7％ vs 100％,P =0.569),over all survival of BRCA1 negative and positive groups was 100％.Conclusions BRCA1 expression may not predict the prognosis of intraductal carcinoma,but ductal carcinoma in situ with microinvasion group ratio in BRCA1 negative was higher than ductal carcinoma in situ group,BRCA1 may take affect within ductal carcinoma infiltration process work.
