Effects of O-GlcNAcylation modification and Akt1 on proliferation and invasion of gastric cancer cells
10.3969/j.issn.1671-8348.2017.08.007
- VernacularTitle:O-GlcNAc糖基化修饰和Akt1对胃癌细胞体外增殖及侵袭力的影响
- Author:
Nuobei ZHANG
;
Xin CHEN
- Keywords:
stomach neoplasms;
neoplasm invasiveness;
cell proliferation;
O-GlcNAcylation;
Akt1
- From:
Chongqing Medicine
2017;46(8):1027-1031,1035
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of O-GlcNAcylation on on proliferation and invasion of gastric cancer cells and evaluate the role of Aktl on O-GlcNAcylation promotting cells proliferation and invasion in gastric cancer.Methods Build the cell model:O-GlcNAc glycosylation levels rise or fall.The cell viability was determine by MTT.To investigate whether O-GlcNAcylation affected colony formation ability of gastric cancer cells,soft agar colony assays were carried out.Cell migration or invasion was using transwell chambers.The expression of Akt1 was detected through Western blot.Thiamet-G was used to eualuate the role of Akt1 on O-Gcnac cylation regulating invasion in gastric Cancei.Results O-GlcNAcylation was increased the gastric cancer cells proliferation ability,colony formation ability,migration and invasion ability in vitro.Akt1 was activated by Ser473 phosphorylation upregulation though O-GlcNAcylation.Akt1 shRNA was inhibition the cell invasive which induced by Thiamet-G.Akt1 overexpression was promoted by Thiamet-G-induced cell invasion.Conclusion O-GlcNAcylation enhanced oncogenic phenotypes possibly partially involving Akt1.
