The role of SPHK-1 in non-small cell lung cancer drug-resistant cell line H460
10.7652/jdyxb201702004
- VernacularTitle:SPHK-1在非小细胞肺癌H460细胞耐药株中的作用
- Author:
Ying GAO
;
Meili TIAN
;
Liping SONG
- Keywords:
non-small cell lung cancer (NSCLC);
sphingosine kinase 1 (SPHK-1 );
sphingosine 1-phosphate (S1 P);
drug resistance
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2017;38(2):172-175,187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the role of SPHK-1/S1P and NFκB p65 signal pathway in non-small cell lung cancer (NSCLC)drug-resistant cells.Methods The drug-resistant cell line of lung cancer H460/DDP was constructed and its biological characteristics were identified successfully.The expression of SPHK-l was tested by RT-PCR and Western blot methods.S1 P and some proteins related to NFκB pathway were studied by Western blot. Results The drug-resistant lung cancer cell line H460/DDP was constructed and its drug-resistant ability was evaluated (IC50H460/DDP = 50.62μg/mL, RIH460/DDP = 2.95 ). Cisplatin at a concentration of 10 - 80 μg/mL significantly decreased cell death of drug-resistant cell line (P<0 .01 ).Western blot assay analysis showed that overexpressions of SPHK-l,S1 P and NFκB p65 were significantly higher in drug-resistant cell line than in their parent cell line H460 (P=0.0415,P=0.0465,P=0.0218).RT-PCR method revealed that SPHK-1 mRNA was overexpressed in drug-resistant cell line compared with that in their parent cell line H460 (P<0.05).More NFκB p65 protein in cell nucleus was expressed in drug-resistant cells than in parent cells.Conclusion SPHK-1/S1P and NFκB p65 signal pathway may play an improtant role in the drug-resistant H460 cell line in non-small cell lung cancer.
