Effects of atorvastatin on expression of lysyl oxidase in myocardial tissue of rats with diabetic cardiomyopathy
10.3969/j.issn.1671-8348.2017.09.006
- VernacularTitle:阿托伐他汀对糖尿病心肌病大鼠心肌组织赖氨酰氧化酶的影响
- Author:
Hui LIU
;
Shuying ZHANG
;
Jidong RONG
- Keywords:
diabetic cardiomyopathy;
protein-lysine 6-oxidese;
matrix metalloproteinase 2;
NF-kappa B;
atorvastatin
- From:
Chongqing Medicine
2017;46(9):1172-1174,1178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of atorvastatin on expression of lysyl oxidase(LOX) in myocardial tissue of rats with diabetic cardiomyopathy (DCM) and its mechanism.Methods Thirty DCM SD rats were randomly divided into 3 groups:DCM group,treatment group (atorvastain 2 mg · kg-1 · d-1 by gastric gavage) and β-aminopropionitrile group(β-aminopropionitrile 80 mg · kg-1 · d-1 by gastric gavage),10 cases in each group.Other 10 SD rats were selected as the control group.At the end of week 8,the rats were killed for extracting the myocardial tissue RNA and protein.Expression levels of LOX,MMP-2 and NF-κB mRNAs and proteins in myocardial tissue of DCM rats were measured by RT-PCR and Western blot.Results The expression levels of LOX,MMP-2 and NF-κB mRNAs and proteins in the DCM group were significantly higher than those in the control group (P＜0.01),and compared with the DCM group,the expression of BAPN LOX,MMP-2 and NF-κB mRNA and proteins in the treatment group were significantly deceased (P＜0.01).Conclusion Atorvastatin can reverse the expression of LOX in myocardial tissue of DCM rat,and then may regulate the expression of MMP-2 and NF-κB.
